ImmuneGuard - Immune Guard - The Ultimate Immune Protection

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L-theanine discovered to be significant immune system booster.

Recently, Dr. Jack Bukowski, MD, PhD, NSRI Chief Scientist, was interviewed on the subject of L-theanine. “As is the case with many discoveries, finding that L-theanine, a major component of tea beverages, boosts the immune system was basically accidental.” admitted, Dr. Jack Bukowski,

Dr. Bukowski had been studying how T cells, an important part of the immune system, are triggered to make interferon gamma, a natural chemical that is crucial to protecting our bodies from viruses, bacteria, and parasites that can cause such illnesses as colds and flu . “T cells are turned on in our immune system by what’s termed a “lock and key” mechanism,” Dr. Bukowski explained. “What I was trying to determine was which part of the lock (receptor) fit with the key (antigen) I was using recombinant DNA technology to engineer small changes into the natural receptors on the T cells, and trying different antigens to turn on the receptor. Unfortunately, I couldn’t seem to find the right combination.”

Although his research initially proved frustrating, Dr. Bukowski remained undaunted. Drawing upon his years of experience as a research scientist at Harvard, he took a new approach. He began to systematically test all the antigens against all the receptors, even the natural, “un-engineered” ones. One of these antigens, called ethylamine, was indeed able to turn on the natural T cell receptor to make interferon gamma.

A new antigen for T cells had been discovered.

Through subsequent experiments, Dr. Bukowski learned that using small amounts of ethylamine antigen allowed the T cells to make lots more interferon gamma to fight foreign bacteria. In other words, it triggered a natural defense mechanism to certain illnesses and conditions. It was definitely an exciting finding…with one notable exception.

The problem was ethylamine is a very basic substance. Right out of the bottle, in pure, concentrated form, it is actually toxic, causing burns and lung damage. Therefore, ethylamine taken orally as a “nutrient” or a “drug” was not an option. “If ethylamine can’t be taken as a supplement, then where does it occur in our diet, since it must get into our body fluids somehow?” became the subsequent mystery that needed to be solved.

Through additional research, Dr. Bukowski learned thathas been known to be present in high concentrations in tea drinkers. How is this possible? In 19 ethylamine 49, a Japanese researcher discovered that tea beverages uniquely contain huge amounts of L-theanine, an amino acid that is broken down in the body to yield ethylamine, the T cell activator needed.

The stage was now set to try a very safe experiment in humans.

The NSRI team, headed by Dr. Bukowski, organized two groups of volunteers:

• 11 non-tea drinking volunteers were asked to consume a certain large quantity of tea, every day for 4 weeks
• 10 volunteers were asked to drink the same amount of coffee for that same amount of time.

Blood samples were taken and everyone’s T cells tested, both before and after the testing began, at one week intervals. It was found that the T cells did make some interferon gamma when exposed to bacteria, even before the tea drinking began. However, astonishingly enough, in a little as one week after the start of the tea drinking, the T cells exposed to bacteria in that testing group made 500% more interferon gamma than they had done the week before! This pattern continued for the entire 4 weeks, in 7 out of the 11 volunteers. In stark contrast, only 1 out of 10 volunteers who drank coffee instead of tea made more interferon gamma.

NSRI had reproduced in humans what was initially found in test tube experiments, an achievement of extreme significant importance as most test tube observations are either not tested in humans or the tests fail altogether.

Clinical Study at the University of Florida

Now, it was time to test the actual Immune Formula in the most rigorous scientific way. Sixty healthy volunteers aged 18-70 were given NSRI’s Immune Guard® for 3 months, and another set of 60 closely matched volunteers were given a placebo sugar pill. Each subject was asked to record the number of days they had cold and flu symptoms in a personal log. They were asked about incidence and duration of headache, stuffy nose, runny nose, cough, sore throat, diarrhea, fever, and how often they needed to see the doctor for these symptoms. The results were very clear: there was a highly significant decrease in the incidence and duration of all these symptoms, in the group taking NSRI’s Immune Guard®, as compared to the group taking placebo. Further, visits to the doctor were decreased by over 50%. Thus NSRI’s Immune Guard® has been clinically proven to decrease the incidence and duration of cold and flu symptoms. These impressive new results have been submitted for publication.

Since, in everyday life, drinking the amount of tea required to yield these impressive benefits would be unrealistic for most Americans, an easy alternative method needed to be developed to achieve the same results. NSRI formulated NSRI’s Immune Guard® with 100 mg L-theanine per capsule, which, when taken twice a day, is the identical amount in the tea given the human volunteers. In addition, NSRI’s Immune Guard® contains EGCG, which acts intimately together with L-theanine to further maximize the immune function of the product. In fact, recent research has shown that NSRI’s Immune Guard® is capable of boosting the immune system by 500-1500%!

Monthly Illnesses and Symptoms for Subjects Taking Immune Guard® and Placebo

*Comparison of number of illness in Immune Guard® and placebo group, month by month, and total
^Comparison of number of symptom days reported in CSF and placebo group, month by month, and total
**48% of all symptom days occurred in February (P<0.002 compared to March or April/May)

Interferon-γ (ng/mL) secreted into the culture medium after 24h

Repeated measures two-way ANOVA.  Means with different superscripts are statistically different using a post hoc SNK test.

% γδ-T cells in baseline blood sample and after 10d of culture

Repeated measures two-way ANOVA; means ± SEM.

1. Kamath AB, Wang L, Das H, Li L Reinhold VN, and Bukowski JF. Antigens in tea beverage prime human Vgamma2 Vdelta2 T cells in vitro and in vivo for memory and nonmemory antibacterial cytokine responses. Proc. Nat. Acad. Sci. 2003. 100:6009-14.
2. Bukowski JF, Morita CT, and Brenner MB. Human gamma delta T cells recognize alkylamines from microbes, tea, and edible plants: implications for innate immunity. Immunity 1999; 11:57-65.
3. Wang L, Kamath A, Das H, Li L, and Bukowski JF. Antibacterial effect of       human Vgamma2 Vdelta2 T cells in vivo. J. Clin. Invest. 2001; 108:1349-1357.